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CRL-10609 MMQ 大鼠垂體瘤細胞

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產品名稱: CRL-10609 MMQ 大鼠垂體瘤細胞
產品型號: CRL-10609
產品廠商: 美國標準生物品收藏中心(ATCC)
產品文檔: 無相關文檔


簡單介紹

CRL-10609 MMQ 大鼠垂體瘤細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*優培養條件!


CRL-10609 MMQ 大鼠垂體瘤細胞 的詳細介紹
CRL-10609 MMQ 大鼠垂體瘤細胞
ATCC® Number: CRL-10609™    Price: $438.00
Designations: MMQ
Depositors:  University of Virginia Patent Foundation
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties:suspension
Organism:Rattus norvegicus (rat)
Morphology:epithelial

Source:Organ: pituitary
Disease: tumor
Cellular Products:prolactin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Receptors:dopamine
Tumorigenic:No
Age: 5 days
Comments:The cells secrete prolactin and express functional dopamine receptors [U.S. Patent 5,510,260].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 2.5%
  • horse serum to a final concentration of 15%
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    DGrowth Conditions: The line grows as clumps of cells in suspension.
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10(5) viable cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 days
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 24 to 48 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
Cell culture tested DMSO:ATCC 4-X
References:22090: MacLeod RM, et al. Prolactin secreting cell line and method of obtaining the same. US Patent 5,510,260 dated Apr 23 1996
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